Seidl, Kati
Goerke, Christiane
Wolz, Christiane
Mack, Dietrich
Berger-Bàˆchi, Brigitte
Bischoff, Markus
<jats:title>ABSTRACT</jats:title>
<jats:p>Biofilm formation in <jats:italic>Staphylococcus aureus</jats:italic> under in vitro growth conditions is generally promoted by high concentrations of sugar and/or salts. The addition of glucose to routinely used complex growth media triggered biofilm formation in <jats:italic>S. aureus</jats:italic> strain SA113. Deletion of <jats:italic>ccpA</jats:italic>, coding for the catabolite control protein A (CcpA), which regulates gene expression in response to the carbon source, abolished the capacity of SA113 to form a biofilm under static and flow conditions, while still allowing primary attachment to polystyrene surfaces. This suggested that CcpA mainly affects biofilm accumulation and intercellular aggregation. <jats:italic>trans</jats:italic>-Complementation of the mutant with the wild-type <jats:italic>ccpA</jats:italic> allele fully restored the biofilm formation. The biofilm produced by SA113 was susceptible to sodium metaperiodate, DNase I, and proteinase K treatment, indicating the presence of polysaccharide intercellular adhesin (PIA), protein factors, and extracellular DNA (eDNA). The investigation of several factors which were reported to influence biofilm formation in <jats:italic>S. aureus</jats:italic> (<jats:italic>arlRS</jats:italic>, <jats:italic>mgrA</jats:italic>, <jats:italic>rbf</jats:italic>, <jats:italic>sarA</jats:italic>, <jats:italic>atl</jats:italic>, <jats:italic>ica</jats:italic>, <jats:italic>citZ</jats:italic>, <jats:italic>citB</jats:italic>, and <jats:italic>cidABC</jats:italic>) showed that CcpA up-regulated the transcription of <jats:italic>cidA</jats:italic>, which was recently shown to contribute to eDNA production. Moreover, we showed that CcpA increased <jats:italic>icaA</jats:italic> expression and PIA production, presumably over the down-regulation of the tricarboxylic acid cycle genes <jats:italic>citB</jats:italic> and <jats:italic>citZ</jats:italic>.</jats:p>
application/pdf
https://sonar.ch/global/documents/103248
eng
info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.00035-08
info:eu-repo/semantics/altIdentifier/issn/0019-9567
info:eu-repo/semantics/openAccess
Infection and Immunity. - American Society for Microbiology. - 2008, vol. 76, no. 5, p. 2044-2050
Immunology
Microbiology
Parasitology
Infectious Diseases
Staphylococcus aureus CcpA Affects Biofilm Formation
http://purl.org/coar/resource_type/c_6501