Poirel, Laurent Kieffer, Nicolas Nordmann, Patrice 2017 <jats:title>ABSTRACT</jats:title> <jats:p>The plasmid-mediated <jats:italic>mcr-1</jats:italic> gene encodes a phosphoethanolamine transferase that confers resistance to polymyxins. The <jats:italic>mcr-1</jats:italic> gene is associated with insertion sequence IS<jats:italic>Apl1</jats:italic> (IS<jats:italic>30</jats:italic> family). <jats:italic>In vitro</jats:italic> mobilization assays demonstrated the functionality of the composite transposon structure IS<jats:italic>Apl1-mcr-1</jats:italic>-IS<jats:italic>Apl1</jats:italic>. Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the <jats:italic>mcr-1</jats:italic> gene by transposition.</jats:p> application/pdf https://sonar.ch/global/documents/106520 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/aac.00127-17 info:eu-repo/semantics/altIdentifier/issn/0066-4804 info:eu-repo/semantics/openAccess Antimicrobial Agents and Chemotherapy. - American Society for Microbiology. - 2017, vol. 61, no. 7 Pharmacology (medical) Pharmacology Infectious Diseases In Vitro Study of ISApl1-Mediated Mobilization of the Colistin Resistance Gene mcr-1 http://purl.org/coar/resource_type/c_6501