Dysli C Dysli M Enzmann V Wolf S Zinkernagel MS 2014 PURPOSE Fundus autofluorescence (AF) is characterized not only by its intensity or excitation and emission spectra but also by the lifetimes of the fluorophores. Fluorescence lifetime is influenced by the fluorophore's microenvironment and may provide information about the metabolic tissue state. We report quantitative and qualitative autofluorescence lifetime imaging of the ocular fundus in mice. METHODS A fluorescence lifetime imaging ophthalmoscope (FLIO) was used to measure fluorescence lifetimes of endogenous fluorophores in the murine retina. FLIO imaging was performed in 1-month-old C57BL/6, BALB/c, and C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. Measurements were repeated at monthly intervals over the course of 6 months. For correlation with structural changes, an optical coherence tomogram was acquired. RESULTS Fundus autofluorescence lifetime images were readily obtained in all mice. In the short spectral channel (498-560 nm), mean ± SEM AF lifetimes were 956 ± 15 picoseconds (ps) in C57BL/6; 801 ± 35 ps in BALB/c mice; and 882 ± 37 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. In the long spectral channel (560-720 nm), mean ± SEM AF lifetimes were 298 ± 14 ps in C57BL/6 mice, 241 ± 10 ps in BALB/c mice, and 288 ± 8 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. There was a general decrease in mean AF lifetimes with age. CONCLUSIONS Although fluorescence lifetime values differ among mouse strains, we found little variance within the groups. Fundus autofluorescence lifetime imaging in mice may provide additional information for understanding retinal disease processes and may facilitate monitoring of therapeutic effects in preclinical studies. https://sonar.ch/global/documents/116459 eng info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.14-14445 info:eu-repo/semantics/altIdentifier/pmid/25249601 Investigative ophthalmology & visual science. - 2014 fluorescence lifetimes fundus autofluorescence mouse models ophthalmic imaging validation studies Animals Fundus Oculi Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Mutant Strains Ophthalmoscopy Optical Imaging Reproducibility of Results Retina Tomography, Optical Coherence Fluorescence lifetime imaging of the ocular fundus in mice. http://purl.org/coar/resource_type/c_6501