<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Eberhard M</dc:creator>
  <dc:creator>Erne P</dc:creator>
  <dc:date>1993</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Metabolism of inositol 1,4,5-trisphosphate (IP3) and IP3-induced release of Ca2+ was analyzed in permeabilized human platelets. Both rapid Ca2+ release and hydrolysis of IP3 to inositol 1,4-bisphosphate (IP2) was observed after addition of IP3 to permeabilized, Ca(2+)-loaded platelets. In the absence of ATP or in the presence of inhibitors of the Ca(2+)-ATPase of the endoplasmic reticulum, no release of Ca2+ and little hydrolysis of IP3 occurs, indicating a coupling between the Ca2+ gradient across the membrane of the IP3-sensitive Ca2+ store and conversion of IP3 to IP2. In addition, the rapid recovery of the sensitivity of the IP3-sensitive Ca2+ store after successive additions of IP3 (increment detection) appears to be associated with hydrolysis of IP3.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/12023</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1006/bbrc.1993.2003</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/8363599</dc:relation>
  <dc:source>Biochemical and biophysical research communications. - 1993</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Aniline Compounds</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Blood Platelets</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Calcium</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Cell Membrane Permeability</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Fluorescent Dyes</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Hydrolysis</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">In Vitro Techniques</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Inositol 1,4,5-Trisphosphate</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Inositol Phosphates</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Kinetics</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Spectrometry, Fluorescence</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Xanthenes</dc:subject>
  <dc:title xmlns:ns14="xml" ns14:lang="en">Inositol 1,4,5-trisphosphate-induced calcium release in permeabilized platelets is coupled to hydrolysis of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>