Beck M Aschwanden M Stemmer A 2008 Combining total internal reflection fluorescence microscopy with structured illumination allows optical wide-field imaging with sub-100-nanometre resolution. We present a novel objective-launch set-up for standing wave illumination that takes advantage of a tunable transmission diffraction grating and transparent phase shifters actuated by electro-active polymers to control the excitation pattern in three dimensions. Image acquisition is completed in less than 1 s. To reconstruct the extended image spectrum, we apply a new apodization function that results in a lateral resolution of 89 nm for green emission wavelength. https://sonar.ch/global/documents/123682 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2818.2008.02075.x info:eu-repo/semantics/altIdentifier/pmid/19017206 Journal of microscopy. - 2008 Pathology and Forensic Medicine Histology Sub-100-nanometre resolution in total internal reflection fluorescence microscopy. http://purl.org/coar/resource_type/c_6501