Furrer P Bednar J Dubochet J Hamiche A Prunell A 1995 Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 on a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 +/- 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region. https://sonar.ch/global/documents/130572 eng info:eu-repo/semantics/altIdentifier/doi/10.1006/jsbi.1995.1017 info:eu-repo/semantics/altIdentifier/pmid/7662486 Journal of structural biology. - 1995 Animals Chromatin Computer Graphics Crystallography, X-Ray DNA Electrophoresis, Polyacrylamide Gel Freezing Histones Image Processing, Computer-Assisted Microscopy, Electron Models, Molecular Nucleic Acid Conformation Nucleosomes DNA at the entry-exit of the nucleosome observed by cryoelectron microscopy. http://purl.org/coar/resource_type/c_6501