Pouly S Matthieu JM Honegger P 2001 In previous work we found that mezerein, a C kinase activator, as well as basic fibroblast growth factor (FGF-2) induce demyelination and partial oligodendrocyte dedifferentiation in highly differentiated aggregating brain cell cultures. Here we show that following protein kinase C activator-induced demyelination, effective remyelination occurs. We found that mezerein or FGF-2 caused a transient increase in DNA synthesis following a pronounced decrease of the myelin markers myelin basic protein and 2',3'-cyclic nucleotide 3'-phosphohydrolase. Both oligodendrocytes and astrocytes were involved in this mitogenic response. Within 17 days after demyelination, myelin was restored to the level of the untreated controls. Transient mitotic activity was indispensable for remyelination. The present results suggest that myelinating oligodendrocytes retain the capacity to reenter the cell cycle, and that this plasticity is important for the regeneration of the oligodendrocyte lineage and remyelination. Although it cannot be excluded that a quiescent population of oligodendrocyte precursor cells was present in the aggregates and able to proliferate, differentiate and remyelinate, we could not find evidence supporting this view. https://sonar.ch/global/documents/139192 eng info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1010931102287 info:eu-repo/semantics/altIdentifier/pmid/11519722 Neurochemical research. - 2001 2',3'-Cyclic-Nucleotide Phosphodiesterases Animals Becaplermin Biomarkers Brain Cell Division Cytarabine DNA Demyelinating Diseases Diterpenes Enzyme Activation Fetus Fibroblast Growth Factor 2 In Vitro Techniques Mitosis Myelin Basic Protein Myelin Sheath Oligodendroglia Platelet-Derived Growth Factor Protein Kinase C Proto-Oncogene Proteins c-sis Rats Terpenes Remyelination in vitro following protein kinase C activator-induced demyelination. http://purl.org/coar/resource_type/c_6501