Raoufi M Das T Schoen I Vogel V Brüggemann D Spatz JP 2015 Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions. https://sonar.ch/global/documents/139882 eng info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.nanolett.5b01356 info:eu-repo/semantics/altIdentifier/pmid/26360649 Nano letters. - 2015 AAO template FRET conformational changes extrusion protein nanofiber Fibronectins Fluorescence Resonance Energy Transfer Microscopy, Electron, Scanning Nanopores Nanopore Diameters Tune Strain in Extruded Fibronectin Fibers. http://purl.org/coar/resource_type/c_6501