Halbeisen RE Scherrer T Gerber AP 2009 We describe ribosome affinity purification (RAP), a method that allows rapid purification of ribosomes and associated messages from the yeast Saccharomyces cerevisiae. The method relies on the expression of protein A tagged versions of the ribosomal protein Rpl16, which is used to efficiently recover endogenously formed ribosomes and polysomes from cellular extracts with IgG-coupled spherical microbeads. This approach can be applied to profile reactions of the translatome, which refers to all messages associated with ribosomes, with those of the transcriptome using DNA microarrays. In addition, ribosomal proteins, their modifications, and/or other associated proteins can be mapped with mass spectrometry. Finally, application of this method in other organisms provides a valuable tool to decipher cell-type specific gene expression patterns. https://sonar.ch/global/documents/142779 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ymeth.2009.04.003 info:eu-repo/semantics/altIdentifier/pmid/19398006 Methods (San Diego, Calif.). - 2009 Cell Fractionation Chromatography, Affinity Protein Array Analysis RNA-Binding Proteins Ribosomal Proteins Ribosomes Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Affinity purification of ribosomes to access the translatome. http://purl.org/coar/resource_type/c_6501