Schönenberger C Schütz A Franco-Obregón A Zenobi-Wong M 2011 Peptide therapeutics are of increasing interest due to their biological specificity. We used a simple technique to study the efficacy of inducing peptides into adherent chondrocytes by transiently permeabilizing the membrane with electric pulses (in situ electroporation). Mechano-growth factor (MGF) was selected as a model peptide. FITC-labeled MGF was added to cultures of adherent primary chondrocytes grown on ITO coated glass slides. Cells were subjected to 3-9 pulses of 175-275 V and evaluated by flow cytometry. Under optimal conditions, an electroporation efficiency of close to 50% could be achieved. This technique can be used to study the functional domains of intracellular peptides, peptide inhibition of signal transduction and intracrine-mediated effects of peptides in adherent cells. application/pdf https://sonar.ch/global/documents/143217 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-010-0508-1 info:eu-repo/semantics/altIdentifier/pmid/21207114 info:eu-repo/semantics/openAccess Biotechnology letters. - 2011 Animals Cattle Cell Adhesion Cells, Cultured Chondrocytes Electroporation Fluorescein-5-isothiocyanate Insulin-Like Growth Factor I Peptides Staining and Labeling Efficient electroporation of peptides into adherent cells: investigation of the role of mechano-growth factor in chondrocyte culture. http://purl.org/coar/resource_type/c_6501