Stücheli P Sieber S Fuchs DW Scheller L Strittmatter T Saxena P Gademann K Fussenegger M 2020 We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian cells. The system does not impair cell viability, and can be used as a non-protein reporter system to directly visualize the dynamics of gene expression by profiling absorbance or fluorescence in the supernatant of cell cultures, as well as for fluorescence labeling of individual cells. Pigment profiling can also be multiplexed with reporter proteins such as mCherry or the human model glycoprotein SEAP (secreted alkaline phosphatase). Furthermore, absorbance measurement with a smartphone camera using standard application software enables inexpensive, low-tech reporter quantification. application/pdf https://sonar.ch/global/documents/144520 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkaa342 info:eu-repo/semantics/altIdentifier/pmid/32421771 info:eu-repo/semantics/openAccess Nucleic acids research. - 2020 Absorption, Radiation Animals CHO Cells Cricetinae Cricetulus Fungal Proteins Genes, Reporter HEK293 Cells Humans Microscopy, Fluorescence Oxygenases Picolinic Acids Recombinant Proteins Single-Cell Analysis Spectrometry, Fluorescence Tyrosine 3-Monooxygenase Ultraviolet Rays Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells. http://purl.org/coar/resource_type/c_6501