Gehre, Lena Gorgette, Olivier Perrinet, Stéphanie Prevost, Marie-Christine Ducatez, Mathieu Giebel, Amanda M Nelson, David E Ball, Steven G Subtil, Agathe 2016 <jats:p>For intracellular pathogens, residence in a vacuole provides a shelter against cytosolic host defense to the cost of limited access to nutrients. The human pathogen Chlamydia trachomatis grows in a glycogen-rich vacuole. How this large polymer accumulates there is unknown. We reveal that host glycogen stores shift to the vacuole through two pathways: bulk uptake from the cytoplasmic pool, and de novo synthesis. We provide evidence that bacterial glycogen metabolism enzymes are secreted into the vacuole lumen through type 3 secretion. Our data bring strong support to the following scenario: bacteria co-opt the host transporter SLC35D2 to import UDP-glucose into the vacuole, where it serves as substrate for de novo glycogen synthesis, through a remarkable adaptation of the bacterial glycogen synthase. Based on these findings we propose that parasitophorous vacuoles not only offer protection but also provide a microorganism-controlled metabolically active compartment essential for redirecting host resources to the pathogens.</jats:p> https://sonar.ch/global/documents/146353 eng info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.12552 info:eu-repo/semantics/altIdentifier/issn/2050-084X eLife. - eLife Sciences Publications, Ltd. - 2016, vol. 5 Sequestration of host metabolism by an intracellular pathogen http://purl.org/coar/resource_type/c_6501