Giampaoli S Alessandrini F Berti A Ripani L Choi A Crab R De Vittori E Egyed B Haas C Lee HY Korabecná M Noel F Podini D Tagliabracci A Valentini A Romano Spica V 2014 Identification of vaginal fluids is an important step in the process of sexual assaults confirmation. Advances in both microbiology and molecular biology defined technical approaches allowing the discrimination of body fluids. These protocols are based on the identification of specific bacterial communities by microfloraDNA (mfDNA) amplification. A multiplex real time-PCR assay (ForFLUID kit) has been developed for identifying biological fluids and for discrimination among vaginal, oral and fecal samples. In order to test its efficacy and reliability of the assay in the identification of vaginal fluids, an interlaboratory evaluation has been performed on homogeneous vaginal swabs. All the involved laboratories were able to correctly recognize all the vaginal swabs, and no false positives were identified when the assay was applied on non-vaginal samples. The assay represents an useful molecular tool that can be easily adopted by forensic geneticists involved in vaginal fluid identification. https://sonar.ch/global/documents/147019 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jflm.2013.10.016 info:eu-repo/semantics/altIdentifier/pmid/24365693 Journal of forensic and legal medicine. - 2014 Bacteria Forensic Interlaboratory evaluation Multiplex PCR Vaginal fluids mfDNA Adult Cervix Mucus DNA, Bacterial Enterococcus faecalis Female Forensic Medicine Humans Laboratories Lactobacillus Multiplex Polymerase Chain Reaction Real-Time Polymerase Chain Reaction Staphylococcus Streptococcus Vagina Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification. http://purl.org/coar/resource_type/c_6501