Krol I Castro-Giner F Maurer M Gkountela S Szczerba BM Scherrer R Coleman N Carreira S Bachmann F Anderson S Engelhardt M Lane H Evans TRJ Plummer R Kristeleit R Lopez J Aceto N 2018 Human glioblastoma (GBM) is a highly aggressive, invasive and hypervascularised malignant brain cancer. Individual circulating tumour cells (CTCs) are sporadically found in GBM patients, yet it is unclear whether multicellular CTC clusters are generated in this disease and whether they can bypass the physical hurdle of the blood-brain barrier.  Here, we assessed CTC presence and composition at multiple time points in 13 patients with progressing GBM during an open-label phase 1/2a study with the microtubule inhibitor BAL101553. We observe CTC clusters ranging from 2 to 23 cells and present at multiple sampling time points in a GBM patient with pleomorphism and extensive necrosis, throughout disease progression. Exome sequencing of GBM CTC clusters highlights variants in 58 cancer-associated genes including ATM, PMS2, POLE, APC, XPO1, TFRC, JAK2, ERBB4 and ALK. Together, our findings represent the first evidence of the presence of CTC clusters in GBM. application/pdf https://sonar.ch/global/documents/150076 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41416-018-0186-7 info:eu-repo/semantics/altIdentifier/pmid/30065256 info:eu-repo/semantics/openAccess British journal of cancer. - 2018 Animals Benzimidazoles Brain Neoplasms Cell Count Cluster Analysis Disease Progression Female Gene Regulatory Networks Genetic Variation Glioblastoma Humans Male Mice Mutation Neoplastic Cells, Circulating Oxadiazoles Whole Exome Sequencing Xenograft Model Antitumor Assays Detection of circulating tumour cell clusters in human glioblastoma. http://purl.org/coar/resource_type/c_6501