Perrin D Koppenol WH 2000 Both peroxynitrous acid and peroxynitrite react with methionine, k(acid) = (1.7 +/- 0.1) x 10(3) M(-1) s(-1) and k(anion) = 8.6 +/- 0.2 M(-1) s(-1), respectively, and with N-acetylmethionine k(acid) = (2.8 +/- 0.1) x 10(3) M(-1) s(-1) and k(anion) = 10.0 +/- 0.1 M(-1) s(-1), respectively, to form sulfoxides. In contrast to the results of Pryor et al. (1994, Proc. Natl. Acad. Sci. USA 91, 11173-11177), a linear correlation between k(obs) and [met] was obtained. Surprisingly, for every two sulfoxides and nitrites formed, one peroxynitrite is converted to nitrate. Thus, methionine also catalyzes the isomerization of peroxynitrite to nitrate. Neither the pH nor the concentration of methionine affected the distribution of the yields of nitrite, nitrate, and methionine sulfoxide, which were the only products detected. No products other than nitrite, nitrate, and methioninesulfoxide could be detected. The reactions of methionine and N-acetylmethionine with peroxynitrous acid and peroxynitrite are simple bimolecular reactions that do not involve an activated form of peroxynitrous acid or of peroxynitrite. Nitrite, produced together with methionine sulfoxide, or present as a contamination in the peroxynitrite preparation, is not innocuous, but oxidizes methionine by one electron, which leads to the formation of methional and ethylene. https://sonar.ch/global/documents/163890 eng info:eu-repo/semantics/altIdentifier/doi/10.1006/abbi.2000.1787 info:eu-repo/semantics/altIdentifier/pmid/10845703 Archives of biochemistry and biophysics. - 2000 Aldehydes Chromatography, High Pressure Liquid Chromatography, Ion Exchange Dose-Response Relationship, Drug Ethylenes Hydrogen-Ion Concentration Kinetics Methionine Models, Chemical Nitrates Nitrites Nitrous Acid Peroxynitrous Acid Spectrophotometry Time Factors The quantitative oxidation of methionine to methionine sulfoxide by peroxynitrite. http://purl.org/coar/resource_type/c_6501