<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Rajendra Y</dc:creator>
  <dc:date>2018</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">We describe a method for polyethyleneimine (PEI) mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for protein expression applicable at scales from 2 mL to 2 L. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of plasmid DNA (pDNA) and PEI to the culture and subsequent incubation at 31 °C with agitation by orbital shaking. This method requires 0.3 mg/L of coding pDNA, 2.7 mg/L of nonspecific (filler) DNA and 15 mg/L of PEI. The production phase is performed at 31 °C in the presence of 0.25% N,N-dimethylacetamide (DMA). We also provide information on culture vessel options, recommended working volumes, and recommended shaking speeds for transfections at scales from 2 mL to 2 L.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/16455</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-8730-6_3</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/30242678</dc:relation>
  <dc:source>Methods in molecular biology (Clifton, N.J.). - 2018</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">CHO cells</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Orbital shaking</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Polyethyleneimine</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Recombinant protein</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Transfection</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">CHO Cells</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Cricetulus</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Polyethyleneimine</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Recombinant Proteins</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Transfection</dc:subject>
  <dc:title xmlns:ns12="xml" ns12:lang="en">PEI-Mediated Transient Gene Expression in CHO Cells.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>