<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Goh S</dc:creator>
  <dc:creator>Ngugi D</dc:creator>
  <dc:creator>Lizundia R</dc:creator>
  <dc:creator>Hostettler I</dc:creator>
  <dc:creator>Woods K</dc:creator>
  <dc:creator>Ballingall K</dc:creator>
  <dc:creator>MacHugh ND</dc:creator>
  <dc:creator>Morrison WI</dc:creator>
  <dc:creator>Weir W</dc:creator>
  <dc:creator>Shiels B</dc:creator>
  <dc:creator>Werling D</dc:creator>
  <dc:date>2016</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://sonar.ch/global/documents/169980</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0162571</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/27611868</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:source>PloS one. - 2016</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Antigens, Protozoan</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">CD8-Positive T-Lymphocytes</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Cattle</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Genes, MHC Class I</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Sheep</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Theileria</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Theileriasis</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Vaccines</dc:subject>
  <dc:title xmlns:ns10="xml" ns10:lang="en">Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>