<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Resink TJ</dc:creator>
  <dc:creator>Tkachuk VA</dc:creator>
  <dc:creator>Bernhardt J</dc:creator>
  <dc:creator>Bühler FR</dc:creator>
  <dc:date>1992</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Atherogenesis is associated with alterations in the properties of different cell types, including monocytes/macrophages (foam cell formation), platelets (increased aggregation), endothelial cells (injury), and smooth muscle cells (SMCs) (lipid accumulation or foam cell formation). Oxidized low density lipoproteins (ox-LDL) play a key role in this vascular pathology. This study investigated the ability of ox-LDL to elicit chemical signaling events in cultured human vascular smooth muscle cells (VSMCs). Ox-LDL was found to stimulate phospholipase C-mediated phosphoinositide turnover in human VSMCs. This response occurred rapidly (within 1 minute) and at low concentrations of ox-LDL (half-maximal effective concentration, approximately 5 micrograms/ml). Ox-LDL-stimulated inositol phosphate accumulation in human VSMCs was inhibited by pretreatment of cells with phorbol 12-myristate 13-acetate and with compounds that elevate cyclic AMP or cyclic GMP. Ca2+ antagonists also blocked the effects of ox-LDL on phosphoinositide turnover. Inhibitors of receptor-endocytotic processes (including receptor clustering, cross-linking, and cytoskeleton-dependent internalization) effectively prevented ox-LDL-induced inositol phosphate generation. The data suggest that ox-LDL promotes phospholipase C-mediated phosphoinositide turnover in a manner analogous to that for other Ca(2+)-mobilizing hormones. The results also support an association between phosphoinositide turnover and receptor-mediated endocytosis. Prevention of the direct effects of ox-LDL on SMCs could prove an interesting therapeutic avenue for the prevention of atherosclerosis.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://sonar.ch/global/documents/170885</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1161/01.atv.12.3.278</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/1312338</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:source>Arteriosclerosis and thrombosis : a journal of vascular biology. - 1992</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Calcium Channel Blockers</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Cells, Cultured</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Cyclic AMP</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Cyclic GMP</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Endocytosis</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Lipoproteins, LDL</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Muscle, Smooth, Vascular</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Oxidation-Reduction</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Phosphatidylinositols</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Second Messenger Systems</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Tetradecanoylphorbol Acetate</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Type C Phospholipases</dc:subject>
  <dc:title xmlns:ns14="xml" ns14:lang="en">Oxidized low density lipoproteins stimulate phosphoinositide turnover in cultured vascular smooth muscle cells.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>