<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Tugulu S</dc:creator>
  <dc:creator>Arnold A</dc:creator>
  <dc:creator>Sielaff I</dc:creator>
  <dc:creator>Johnsson K</dc:creator>
  <dc:creator>Klok HA</dc:creator>
  <dc:date>2005</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/173672</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/bm050016n</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/15877383</dc:relation>
  <dc:source>Biomacromolecules. - 2005</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Polymers</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Protein Binding</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Proteins</dc:subject>
  <dc:title xmlns:ns4="xml" ns4:lang="en">Protein-functionalized polymer brushes.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>