Mandriota SJ Seghezzi G Vassalli JD Ferrara N Wasi S Mazzieri R Mignatti P Pepper MS 1995 Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and endothelial cell-specific mitogen that stimulates urokinase-type plasminogen activator (uPA) activity in vascular endothelial cells. Here, we report that VEGF increases the high affinity binding of uPA to the same cells and that this binding is prevented by a peptide corresponding to the uPA receptor (uPAR) binding growth factor-like domain of uPA. Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. By Scatchard analysis, VEGF increased the number of uPAR molecules by 2.8-3.5-fold and concomitantly decreased their affinity for uPA. By northern blotting uPAR mRNA was increased in a dose- and time-dependent manner in response to VEGF. Taken together, these findings demonstrate that VEGF-induced angiogenesis is accompanied by increased uPAR expression and uPA activity on the endothelial cell surface. These observations are consistent with the notion that the uPA-uPAR interaction facilitates cellular invasion. application/pdf https://sonar.ch/global/documents/174925 eng info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.270.17.9709 info:eu-repo/semantics/altIdentifier/pmid/7730348 info:eu-repo/semantics/openAccess The Journal of biological chemistry. - 1995 Animals Cattle Cells, Cultured Endothelial Growth Factors Endothelium, Vascular Enzyme Activation Lymphatic System Lymphokines Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases RNA, Messenger Receptors, Cell Surface Receptors, Urokinase Plasminogen Activator Urokinase-Type Plasminogen Activator Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Vascular endothelial growth factor increases urokinase receptor expression in vascular endothelial cells. http://purl.org/coar/resource_type/c_6501