Menoud PA Sappino N Boudal-Khoshbeen M Vassalli JD Sappino AP 1996 The serpin alpha2-antiplasmin (alpha2-AP) is the major circulating inhibitor of plasmin; it plays a determining role in the regulation of intravascular fibrinolysis, In addition to blood plasma, plasmin formation occurs in various organs where it is thought to fulfill a spectrum of functions not restricted to clot lysis. Alpha2-AP is synthesized by hepatocytes, but other possible sites of production have not been investigated. To explore the potential extravascular contribution of alpha2-AP in the regulation of proteolysis, we have isolated the murine alpha2-AP cDNA and determined its mRNA distribution in adult tissues. In addition to liver, kidneys are major sites of alpha2-AP mRNA accumulation in the mouse. The transcript is present in epithelial cells lining the convoluted portion of proximal tubules, and its accumulation is under androgen control. Human kidneys also contain high levels of alpha2-AP mRNA. Moderate amounts Of alpha2-AP mRNA are detected in other murine tissues such as muscle, intestine, central nervous system, and placenta. Our observations indicate that alpha2-AP can be synthesized in a number of tissues, where it could function as a distal regulator of plasmin-mediated extracellular proteolysis. application/pdf https://sonar.ch/global/documents/175036 eng info:eu-repo/semantics/altIdentifier/doi/10.1172/JCI118694 info:eu-repo/semantics/altIdentifier/pmid/8647939 info:eu-repo/semantics/openAccess The Journal of clinical investigation. - 1996 Amino Acid Sequence Animals Cattle Cloning, Molecular Female Humans Kidney Liver Male Mice Mice, Inbred Strains Molecular Sequence Data Orchiectomy Organ Specificity Pregnancy RNA, Messenger Sequence Homology, Amino Acid Tissue Plasminogen Activator Transcription, Genetic Urokinase-Type Plasminogen Activator alpha-2-Antiplasmin The kidney is a major site of alpha(2)-antiplasmin production. http://purl.org/coar/resource_type/c_6501