Arquint C Nigg EA 2016 Centrioles are microtubule-based core components of centrosomes and cilia. They are duplicated exactly once during S-phase progression. Central to formation of each new (daughter) centriole is the formation of a nine-fold symmetrical cartwheel structure onto which microtubule triplets are deposited. In recent years, a module comprising the protein kinase polo-like kinase 4 (PLK4) and the two proteins STIL and SAS-6 have been shown to stay at the core of centriole duplication. Depletion of any one of these three proteins blocks centriole duplication and, conversely, overexpression causes centriole amplification. In this short review article, we summarize recent insights into how PLK4, STIL and SAS-6 co-operate in space and time to form a new centriole. These advances begin to shed light on the very first steps of centriole biogenesis. application/pdf https://sonar.ch/global/documents/17732 eng info:eu-repo/semantics/altIdentifier/doi/10.1042/BST20160116 info:eu-repo/semantics/altIdentifier/pmid/27911707 info:eu-repo/semantics/openAccess Biochemical Society transactions. - 2016 PLK4 SAS-6 cartwheel formation centriole duplication centrosomes Amino Acid Sequence Animals Cell Cycle Proteins Centrioles Humans Intracellular Signaling Peptides and Proteins Models, Biological Protein Binding Protein-Serine-Threonine Kinases Sequence Homology, Amino Acid The PLK4-STIL-SAS-6 module at the core of centriole duplication. http://purl.org/coar/resource_type/c_6501