Pircher H Müller KP Kyewski BA Hengartner H 1992 Clonal deletion of thymocytes bearing TCR for self antigens is one major mechanism of T cell tolerance induction. Peptide antigen-induced deletion of thymocytes from alpha beta TCR transgenic mice has been studied using single cell suspension cultures. The results show that antigen-presenting immature CD4+CD8+ thymocytes can tolerize antigen-reactive immature thymocytes in vitro by programmed cell death (apoptosis) 6-8 h after antigen exposure. Antigen-induced apoptosis of immature thymocytes was inhibited by antibodies specific for the alpha beta TCR, CD3, CD8, and LFA-1 molecules. This implies that clonal elimination of self-reactive CD4+CD8+ thymocytes does not depend on specialized deleting cell types in the thymus and occurs whenever the TCR of immature thymocytes bind antigen fragments presented by MHC molecules. application/pdf https://sonar.ch/global/documents/185370 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/intimm/4.9.1065 info:eu-repo/semantics/altIdentifier/pmid/1390437 info:eu-repo/semantics/openAccess International immunology. - 1992 Amino Acid Sequence Animals Apoptosis CD4 Antigens CD8 Antigens Cells, Cultured Immune Tolerance Lymphocytic choriomeningitis virus Mice Mice, Transgenic Molecular Sequence Data Receptors, Antigen, T-Cell T-Lymphocytes Viral Proteins Thymocytes can tolerize thymocytes by clonal deletion in vitro. http://purl.org/coar/resource_type/c_6501