<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Hunziker PE</dc:creator>
  <dc:creator>Sternlieb I</dc:creator>
  <dc:date>1991</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">We studied the Cu-MT present in the hepatic cytosol obtained from 7 patients suffering from conditions associated with hepatic Cu overload (Wilson's disease, biliary atresia, familial cholestatic cirrhosis). Since chromatographic methods appropriate for the isolation of Zn- and Cd-MT were unsuitable for Cu-MT, we developed an indirect procedure for the estimation and resolution of the latter. This procedure involved the preparation of apo-MT and its reconstitution to holo-MT with Zn or Cd. Three predominant isoforms of MT were present in all specimens. Our results indicate that at most 36 +/- 5% of the Cu present in the 10 kDa fraction of cytosol is bound to MT in the liver of patients with hepatic copper overload.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/185394</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2362.1991.tb01396.x</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/1752285</dc:relation>
  <dc:source>European journal of clinical investigation. - 1991</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Adolescent</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Adult</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Biliary Atresia</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Child</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Child, Preschool</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Copper</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Cytosol</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Female</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Hepatolenticular Degeneration</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Liver</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Liver Cirrhosis, Biliary</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Male</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Metallothionein</dc:subject>
  <dc:title xmlns:ns15="xml" ns15:lang="en">Copper metallothionein in patients with hepatic copper overload.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>