Kupper U Linden M Cao KZ Lerch K 1990 Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is only expressed in situations of starvation or sexual differentiation. Complete integration of a 400 bp metallothionein promoter-fragment leads to constitutive expression of protyrosinase, whereas a 3.6 kb promoter-fragment conferred copper inducibility on the reporter gene in four transformants. A transformant with high constitutive tyrosinase levels was able to produce melanin on complete medium agar plates supplemented with 1 mg/ml L-tyrosine. https://sonar.ch/global/documents/185422 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00318214 info:eu-repo/semantics/altIdentifier/pmid/2147580 Current genetics. - 1990 Cloning, Molecular Copper DNA, Fungal Enzyme Induction Enzyme Precursors Gene Expression Regulation, Fungal Genes, Fungal Melanins Metallothionein Monophenol Monooxygenase Neurospora crassa Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Recombinant Fusion Proteins Transformation, Genetic Expression of tyrosinase in vegetative cultures of Neurospora crassa transformed with a metallothionein promoter/protyrosinase fusion gene. http://purl.org/coar/resource_type/c_6501