<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Pinol MR</dc:creator>
  <dc:creator>Kägi U</dc:creator>
  <dc:creator>Heizmann CW</dc:creator>
  <dc:creator>Vogel B</dc:creator>
  <dc:creator>Séquier JM</dc:creator>
  <dc:creator>Haas W</dc:creator>
  <dc:creator>Hunziker W</dc:creator>
  <dc:date>1990</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Many processes in the CNS depend on calcium. The calcium signal is transduced into an intracellular response via Ca2(+)-binding proteins, including calbindin D-28K. In many laboratories, polyclonal antibodies against chicken intestinal calbindin D-28K have been used to study its localization in the brain (normal and degenerated) of various species, including humans, but some of these antisera cross-reacted with other proteins, including calretinin. We purified recombinant rat brain calbindin D-28K to raise antisera in rabbits and purified a recombinant rat-chicken calbindin D-28K hybrid protein to immunize mice for the generation of monoclonal antibodies. These antisera were highly specific for calbindin D-28K, as demonstrated by two-dimensional Western blotting analysis. Immunohistochemical analyses combined with in situ hybridization studies demonstrated that calbindin D-28K in the Purkinje cells of the cerebellum is independent of vitamin D. The antibodies described here will be important tools for studying the regulation of expression of calbindin D-28K and its biological function in the brain and in the PNS.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/185436</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.1990.tb04879.x</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/2338543</dc:relation>
  <dc:source>Journal of neurochemistry. - 1990</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Antibodies</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Antibodies, Monoclonal</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Antibody Specificity</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Brain</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Calbindins</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Chickens</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">DNA</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Immune Sera</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Immunohistochemistry</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Kidney</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Nucleic Acid Hybridization</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Rats</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Rats, Inbred Strains</dc:subject>
  <dc:subject xmlns:ns15="xml" ns15:lang="en">Recombinant Proteins</dc:subject>
  <dc:subject xmlns:ns16="xml" ns16:lang="en">S100 Calcium Binding Protein G</dc:subject>
  <dc:subject xmlns:ns17="xml" ns17:lang="en">Tissue Distribution</dc:subject>
  <dc:subject xmlns:ns18="xml" ns18:lang="en">Vitamin D Deficiency</dc:subject>
  <dc:title xmlns:ns19="xml" ns19:lang="en">Poly- and monoclonal antibodies against recombinant rat brain calbindin D-28K were produced to map its selective distribution in the central nervous system.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>