Markovich D Forgo J Stange G Biber J Murer H 1993 Injection of rat kidney cortex mRNA into Xenopus laevis oocytes leads to a stimulation of Na(+)-dependent SO4(2-) uptake. Based on this information, we have isolated from a corresponding library a cDNA (NaSi-1) that is most likely related to a Na+/SO4(2-) cotransport system. NaSi-1 cRNA leads in a time- and dose-dependent manner to specific stimulation of Na(+)-dependent SO4(2-) uptake in oocytes. The apparent affinity constants of the NaSi-1 cRNA-expressed transport resemble those of Na+/SO4(2-) cotransport in brush-border membrane. The NaSi-1 cDNA contains 2239 bp [including a poly(A) tail] and encodes a protein of 595 amino acids (66.05 kDa); the hydropathy profile suggests at least eight membrane-spanning regions. In vitro translation of NaSi-1 cRNA results in a protein of the expected size and suggests glycosylation. Northern blot analysis shows signals of 2.3 and 2.9 kb in kidney (more abundant in cortex than in papilla/medulla) and in mucosa of small intestine of rats. The above data indicate that we have structurally identified a membrane protein involved in renal and small-intestinal brush-border membrane Na+/SO4(2-) cotransport. application/pdf https://sonar.ch/global/documents/185529 eng info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.90.17.8073 info:eu-repo/semantics/altIdentifier/pmid/7690140 info:eu-repo/semantics/openAccess Proceedings of the National Academy of Sciences of the United States of America. - 1993 Amino Acid Sequence Animals Base Sequence Blotting, Northern Carrier Proteins Cation Transport Proteins Cloning, Molecular DNA Female Gene Library Kidney Cortex Kinetics Molecular Sequence Data Oligonucleotides, Antisense Oocytes Organ Specificity Poly A Protein Biosynthesis Protein Conformation RNA RNA, Messenger Rats Sodium Sodium Sulfate Cotransporter Sulfates Symporters Xenopus laevis Expression cloning of rat renal Na+/SO4(2-) cotransport. http://purl.org/coar/resource_type/c_6501