<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Lara E</dc:creator>
  <dc:creator>Fernández LD</dc:creator>
  <dc:creator>Schiaffino MR</dc:creator>
  <dc:creator>Izaguirre I</dc:creator>
  <dc:date>2017</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene+ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/186446</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejop.2017.05.008</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/28662491</dc:relation>
  <dc:source>European journal of protistology. - 2017</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Mamiellophyceae</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Picoplankton</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Ribosomal genes</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Southern Patagonia</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Chlorophyta</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">DNA, Ribosomal</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">DNA, Ribosomal Spacer</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Lakes</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Metagenomics</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Phylogeny</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Species Specificity</dc:subject>
  <dc:title xmlns:ns12="xml" ns12:lang="en">First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>