Denichenko P Mogilevsky M Cléry A Welte T Biran J Shimshon O Barnabas GD Danan-Gotthold M Kumar S Yavin E Levanon EY Allain FH Geiger T Levkowitz G Karni R 2019 Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of a RNA motif, which is recognized by a single SF. Here we show that decoy oligonucleotides targeting splicing factors RBFOX1/2, SRSF1 and PTBP1, can specifically bind to their respective SFs and inhibit their splicing and biological activities both in vitro and in vivo. These decoy oligonucleotides present an approach to specifically downregulate SF activity in conditions where SFs are either up-regulated or hyperactive. application/pdf https://sonar.ch/global/documents/188393 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-019-09523-0 info:eu-repo/semantics/altIdentifier/pmid/30962446 info:eu-repo/semantics/openAccess Nature communications. - 2019 Alternative Splicing Animals Animals, Genetically Modified Binding Sites Glioblastoma HEK293 Cells Heterogeneous-Nuclear Ribonucleoproteins Humans MAP Kinase Signaling System Muscle, Skeletal Nonsense Mediated mRNA Decay Oligonucleotides Polypyrimidine Tract-Binding Protein RNA Splicing Factors Serine-Arginine Splicing Factors Tandem Repeat Sequences Xenograft Model Antitumor Assays Zebrafish Specific inhibition of splicing factor activity by decoy RNA oligonucleotides. http://purl.org/coar/resource_type/c_6501