Finke D Acha-Orbea H Mattis A Lipp M Kraehenbuhl J 2002 CD4+CD3- cells are the predominant hematopoietic cells found in mouse fetal intestine. We prove their role as Peyer's patch (PP)-inducing cells by transfer into neonatal PP-deficient mice. To test the requirement of chemokines and adhesion molecules in induction of PP, we studied mice deficient in CXCR5 and/or alpha4beta1 integrin-mediated adhesion. CXCR5-/- mice have CD4+CD3- cells, which are inefficient in inducing PP formation. We show here that CXCR5/CXCL13 signaling activates alpha4beta1 integrin on CD4+CD3- cells. Blocking of beta1 integrin or VCAM-1, the ligand of alpha4beta1 integrin, inhibits PP formation. This study demonstrates the link between chemokine receptors and adhesion molecules that regulates stromal/hematopoietic cell interaction leading to PP formation. https://sonar.ch/global/documents/189473 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/s1074-7613(02)00395-3 info:eu-repo/semantics/altIdentifier/pmid/12354388 Immunity. - 2002 Adoptive Transfer Animals CD3 Complex CD4-Positive T-Lymphocytes Cell Adhesion Chemokine CXCL13 Chemokines, CXC Immunologic Deficiency Syndromes Integrin alpha4beta1 Mice Mice, Inbred C57BL Mice, Knockout Models, Immunological Morphogenesis Peyer's Patches Radiation Chimera Receptors, CXCR5 Receptors, Chemokine Receptors, Cytokine Signal Transduction Stromal Cells Vascular Cell Adhesion Molecule-1 CD4+CD3- cells induce Peyer's patch development: role of alpha4beta1 integrin activation by CXCR5. http://purl.org/coar/resource_type/c_6501