Kobayashi T Beuchat MH Chevallier J Makino A Mayran N Escola JM Lebrand C Cosson P Kobayashi T Gruenberg J 2002 Very little is known about the biophysical properties and the lipid or protein composition of membrane domains presumably present in endocytic and biosynthetic organelles. Here we analyzed the membrane composition of late endosomes by suborganellar fractionation in the absence of detergent. We found that the internal membranes of this multivesicular organelle can be separated from the limiting membrane and that each membrane population exhibited a defined composition. Our data also indicated that internal membranes may consist of at least two populations, containing primarily phosphatidylcholine or lysobisphosphatidic acid as major phospholipid, arguing for the existence of significant microheterogeneity within late endosomal membranes. We also found that lysobisphosphatidic acid exhibited unique pH-dependent fusogenic properties, and we speculated that this lipid is an ideal candidate to regulate the dynamic properties of this internal membrane mosaic. application/pdf https://sonar.ch/global/documents/195534 eng info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M202838200 info:eu-repo/semantics/altIdentifier/pmid/12065580 info:eu-repo/semantics/openAccess The Journal of biological chemistry. - 2002 Animals Cell Line Cricetinae Endosomes Fatty Acids, Nonesterified Hydrogen-Ion Concentration Intracellular Membranes Kidney Lysophospholipids Membrane Fusion Membrane Lipids Monoglycerides Phospholipids Separation and characterization of late endosomal membrane domains. http://purl.org/coar/resource_type/c_6501