Kuzmenko ES Djafarzadeh S Cakar ZP Fiedler K 2004 Endothelial cell (EC) cultures of different, selected vascular beds and/or organs were screened for receptor-mediated transport of proteins with a semipermeable filter assay. In SVEC4-10 cells, a mouse lymphoid endothelial cell line, orosomucoid, albumin, insulin and LDL were transcytosed from the apical (luminal) to basal (abluminal) side by a receptor-mediated pathway. Specific LDL transcytosis involved transport of intact LDL. A pathway of degradation of LDL and basal release involved vesicles in transport to lysosomes and amino acid merocrine secretion. This newly described transcellular passage of LDL via lysosomes, as well as the standard pathway, were reduced to 70% by PEG(50)-cholesterol (PEG-Chol). Combined results of temperature-dependence analysis and PEG(50)-cholesterol sensitivity show that two pathways contribute to general LDL transcellular passage. We suggest a mechanism of domain hopping by protein membrane diffusion of receptors as the pathway for intact LDL delivery. Based on theoretical considerations we propose that active transport by protein membrane diffusion can be facilitated by an organizational structure of lipid microdomains and polar cellular organization. https://sonar.ch/global/documents/198505 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocel.2003.09.010 info:eu-repo/semantics/altIdentifier/pmid/14687929 The international journal of biochemistry & cell biology. - 2004 Animals Biological Transport Carrier Proteins Cell Line Cell Membrane Cholesterol Cholesterol, LDL Diffusion Endothelial Cells Golgi Matrix Proteins Lysosomes Membrane Proteins Mice Microscopy, Electron, Scanning Polyethylene Glycols Receptors, Lipoprotein Vesicular Transport Proteins LDL transcytosis by protein membrane diffusion. http://purl.org/coar/resource_type/c_6501