Reina JH Azzouz TN Hernandez N 2006 BACKGROUND Human RNA polymerase III (pol III) transcription is regulated by several factors, including the tumor suppressors P53 and Rb, and the proto-oncogene c-Myc. In yeast, which lacks these proteins, a central regulator of pol III transcription, called Maf1, has been described. Maf1 is required for repression of pol III transcription in response to several signal transduction pathways and is broadly conserved in eukaryotes. METHODOLOGY/PRINCIPAL FINDINGS We show that human endogenous Maf1 can be co-immunoprecipitated with pol III and associates in vitro with two pol III subunits, the largest subunit RPC1 and the alpha-like subunit RPAC2. Maf1 represses pol III transcription in vitro and in vivo and is required for maximal pol III repression after exposure to MMS or rapamycin, treatments that both lead to Maf1 dephosphorylation. CONCLUSIONS/SIGNIFICANCE These data suggest that Maf1 is a major regulator of pol III transcription in human cells. application/pdf https://sonar.ch/global/documents/19926 eng info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0000134 info:eu-repo/semantics/altIdentifier/pmid/17205138 info:eu-repo/semantics/openAccess PloS one. - 2006 Base Sequence Cell Line DNA Primers HeLa Cells Humans In Vitro Techniques Methyl Methanesulfonate Phosphorylation Promoter Regions, Genetic Protein Subunits RNA Interference RNA Polymerase I RNA Polymerase II RNA Polymerase III RNA Stability RNA, Messenger RNA, Transfer Recombinant Fusion Proteins Repressor Proteins TATA-Binding Protein Associated Factors Transcription, Genetic Maf1, a new player in the regulation of human RNA polymerase III transcription. http://purl.org/coar/resource_type/c_6501