Ackema KB Hench J Böckler S Wang SC Sauder U Mergentaler H Westermann B Bard F Frank S Spang A 2014 The small GTPase Arf1 plays critical roles in membrane traffic by initiating the recruitment of coat proteins and by modulating the activity of lipid-modifying enzymes. Here, we report an unexpected but evolutionarily conserved role for Arf1 and the ArfGEF GBF1 at mitochondria. Loss of function of ARF-1 or GBF-1 impaired mitochondrial morphology and activity in Caenorhabditis elegans. Similarly, mitochondrial defects were observed in mammalian and yeast cells. In Saccharomyces cerevisiae, aberrant clusters of the mitofusin Fzo1 accumulated in arf1-11 mutants and were resolved by overexpression of Cdc48, an AAA-ATPase involved in ER and mitochondria-associated degradation processes. Yeast Arf1 co-fractionated with ER and mitochondrial membranes and interacted genetically with the contact site component Gem1. Furthermore, similar mitochondrial abnormalities resulted from knockdown of either GBF-1 or contact site components in worms, suggesting that the role of Arf1 in mitochondrial functioning is linked to ER-mitochondrial contacts. Thus, Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic. application/pdf https://sonar.ch/global/documents/201722 eng info:eu-repo/semantics/altIdentifier/doi/10.15252/embj.201489039 info:eu-repo/semantics/altIdentifier/pmid/25190516 info:eu-repo/semantics/openAccess The EMBO journal. - 2014 Cdc48 ER–mitochondrial contact sites mitochondria‐associated degradation mitophagy stress response ADP-Ribosylation Factor 1 Animals Caenorhabditis elegans Caenorhabditis elegans Proteins GTP Phosphohydrolases Membrane Proteins Mitochondria Mitochondrial Membranes Mitochondrial Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins The small GTPase Arf1 modulates mitochondrial morphology and function. http://purl.org/coar/resource_type/c_6501