Mauvoisin D Atger F Dayon L Núñez Galindo A Wang J Martin E Da Silva L Montoliu I Collino S Martin FP Ratajczak J Cantó C Kussmann M Naef F Gachon F 2017 Lysine acetylation is involved in various biological processes and is considered a key reversible post-translational modification in the regulation of gene expression, enzyme activity, and subcellular localization. This post-translational modification is therefore highly relevant in the context of circadian biology, but its characterization on the proteome-wide scale and its circadian clock dependence are still poorly described. Here, we provide a comprehensive and rhythmic acetylome map of the mouse liver. Rhythmic acetylated proteins showed subcellular localization-specific phases that correlated with the related metabolites in the regulated pathways. Mitochondrial proteins were over-represented among the rhythmically acetylated proteins and were highly correlated with SIRT3-dependent deacetylation. SIRT3 activity being nicotinamide adenine dinucleotide (NAD)+ level-dependent, we show that NAD+ is orchestrated by both feeding rhythms and the circadian clock through the NAD+ salvage pathway but also via the nicotinamide riboside pathway. Hence, the diurnal acetylome relies on a functional circadian clock and affects important diurnal metabolic pathways in the mouse liver. application/pdf https://sonar.ch/global/documents/20287 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2017.07.065 info:eu-repo/semantics/altIdentifier/pmid/28813682 info:eu-repo/semantics/openAccess Cell reports. - 2017 NAD(+) SILAC proteomics SIRT3 acetylation circadian clock liver metabolism ARNTL Transcription Factors Acetylation Animals Circadian Clocks Circadian Rhythm Cryptochromes Eating Liver Lysine Metabolic Networks and Pathways Mice Mice, Inbred C57BL Mice, Knockout Mitochondrial Proteins NAD Photoperiod Protein Processing, Post-Translational Proteome Sirtuin 3 Circadian and Feeding Rhythms Orchestrate the Diurnal Liver Acetylome. http://purl.org/coar/resource_type/c_6501