<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Ehrengruber MU</dc:creator>
  <dc:creator>Lundstrom K</dc:creator>
  <dc:date>2002</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Semliki Forest virus (SFV) and Sindbis virus (SIN) are two, positive-strand RNA viruses of the alphavirus genus. Vectors for both have been developed to express high levels of foreign genes in vitro and in vivo. Basic Protocol 1 describes the preparation of packaged SFV and SIN replicons by co-electroporation of helper and vector RNA into baby hamster kidney (BHK)-21 cells. Basic Protocol 2 describes the activation of packaged SFV replicons with a-chymotrypsin. Basic Protocol 3 provides a method for the infection of hippocampal slices. Basic Protocol 4 is a technique for the infection of primary cultures of dispersed neurons with infectious SFV and SIN replicons. The Alternate Protocol describes a method for the cotransfection of in vitro-transcribed vector and helper RNA into BHK-21 cells. Support Protocol 1 describes determining the titers of infectious SFV and SIN replicon stocks, and Support Protocol 2 for metabolic labeling of infected cells.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/203942</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1002/0471142905.hg1202s33</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/18428324</dc:relation>
  <dc:source>Current protocols in human genetics. - 2002</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Cell Line</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Cricetinae</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Genetic Vectors</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Genetics, Medical</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Hippocampus</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Neurons</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Plasmids</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Rats</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Replicon</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Semliki forest virus</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Sindbis Virus</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Tissue Culture Techniques</dc:subject>
  <dc:subject xmlns:ns15="xml" ns15:lang="en">Transfection</dc:subject>
  <dc:subject xmlns:ns16="xml" ns16:lang="en">Virus Assembly</dc:subject>
  <dc:title xmlns:ns17="xml" ns17:lang="en">Semliki Forest virus and Sindbis virus vectors.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>