Hergovich A Lamla S Nigg EA Hemmings BA 2007 Human NDR kinases are upregulated in some cancer types, yet their functions still remain undefined. Here, we report the first known function of a mammalian NDR kinase by demonstrating that human NDR directly contributes to centrosome duplication. A subpopulation of endogenous NDR localizes to centrosomes in a cell-cycle-dependent manner. Overexpression of NDR resulted in centrosome overduplication in a kinase-activity-dependent manner, while expression of kinase-dead NDR or depletion of NDR by small interfering RNA (siRNA) negatively affected centrosome duplication. By targeting NDR to the centrosome, we show that the centrosomal pool of NDR is sufficient to generate supernumerary centrosomes. Furthermore, our data indicate that NDR-driven centrosome duplication requires Cdk2 activity and that Cdk2-induced centrosome amplification is affected upon reduction of NDR activity. Overall, considering that centrosome overduplication is linked to cellular transformation, our observations may also provide a molecular link between mammalian NDR kinases and cancer. https://sonar.ch/global/documents/205482 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcel.2007.01.020 info:eu-repo/semantics/altIdentifier/pmid/17317633 Molecular cell. - 2007 Animals CHO Cells COS Cells Centrosome Chlorocebus aethiops Cricetinae Cricetulus Cyclin-Dependent Kinase 2 Gene Expression HCT116 Cells HeLa Cells Humans Mice NIH 3T3 Cells Protein Binding Protein Transport Protein-Serine-Threonine Kinases Centrosome-associated NDR kinase regulates centrosome duplication. http://purl.org/coar/resource_type/c_6501