<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Volpetti F</dc:creator>
  <dc:creator>Petrova E</dc:creator>
  <dc:creator>Maerkl SJ</dc:creator>
  <dc:date>2017</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between an engineered cellular system, environment, and user. We created a biodisplay consisting of 768 individually programmable biopixels and demonstrated that it can perform multiplexed, continuous sampling. The biodisplay detected 10 μg/L sodium-arsenite in tap water using a research grade fluorescent microscope, and reported arsenic contamination down to 20 μg/L with an easy to interpret "skull and crossbones" symbol detectable with a low-cost USB microscope or by eye. The biodisplay was designed to prevent release of chemical or biological material to avoid environmental contamination. The microfluidic biodisplay thus provides a practical solution for the deployment and application of engineered cellular systems.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/215208</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/acssynbio.7b00088</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/28771313</dc:relation>
  <dc:source>ACS synthetic biology. - 2017</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">arsenic</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">biodisplay</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">continuous bacterial culturing</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">environmental sensor</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">heavy metal sensor</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">microfluidics</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Bacillus subtilis</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Cell Engineering</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Escherichia coli</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Lab-On-A-Chip Devices</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Microfluidic Analytical Techniques</dc:subject>
  <dc:title xmlns:ns12="xml" ns12:lang="en">A Microfluidic Biodisplay.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>
