Burda P Aebi M 1999 The oligosaccharide substrate for the N-linked protein glycosylation is assembled at the membrane of the endoplasmic reticulum. Dolichyl pyrophosphate serves as a carrier in this biosynthetic pathway. In this review, we discuss the function of the lipid carrier dolichol in oligosaccharide assembly and give an overview of the biosynthesis of the different sugar donors required for the building of the oligosaccharide. Yeast genetic techniques have made it possible to identify many different loci encoding specific glycosyltransferases required for the precise and ordered assembly of the dolichyl pyrophosphate-linked oligosaccharide. Based on the knowledge obtained from studying this pathway in yeast, we compare it to the process of N-linked protein glycosylation in archaea. We suggest that N-linked glycosylation in eukaryotes and in archaea share a common evolutionary origin. https://sonar.ch/global/documents/222177 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/s0304-4165(98)00127-5 info:eu-repo/semantics/altIdentifier/pmid/9878760 Biochimica et biophysica acta. - 1999 Amino Acid Sequence Carbohydrate Sequence Dolichol Phosphates Evolution, Molecular Glycoproteins Glycosylation Glycosyltransferases Intracellular Membranes Lipopolysaccharides Molecular Sequence Data Nitrogen Oligosaccharides Yeasts The dolichol pathway of N-linked glycosylation. http://purl.org/coar/resource_type/c_6501