Ravichandran M Priebe S Grigolon G Rozanov L Groth M Laube B Guthke R Platzer M Zarse K Ristow M 2018 Whether and how regulation of genes and pathways contributes to physiological aging is topic of intense scientific debate. By performing an RNA expression-based screen for genes downregulated during aging of three different species, we identified glycine-C-acetyltransferase (GCAT, EC 2.3.1.29). Impairing gcat expression promotes the lifespan of C. elegans by interfering with threonine catabolism to promote methylglyoxal (MGO; CAS 78-98-8) formation in an amine oxidase-dependent manner. MGO is a reactive dicarbonyl inducing diabetic complications in mammals by causing oxidative stress and damaging cellular components, including proteins. While high concentrations of MGO consistently exert toxicity in nematodes, we unexpectedly find that low-dose MGO promotes lifespan, resembling key mediators of gcat impairment. These were executed by the ubiquitin-proteasome system, namely PBS-3 and RPN-6.1 subunits, regulated by the stress-responsive transcriptional regulators SKN-1/NRF2 and HSF-1. Taken together, GCAT acts as an evolutionary conserved aging-related gene by orchestrating an unexpected nonlinear impact of proteotoxic MGO on longevity. application/pdf https://sonar.ch/global/documents/223724 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cmet.2018.02.004 info:eu-repo/semantics/altIdentifier/pmid/29551589 info:eu-repo/semantics/openAccess Cell metabolism. - 2018 aging diabetes healthspan hormesis lifespan longevity metabolism proteostasis proteotoxicity reactive oxygen species Acetyltransferases Animals Caenorhabditis elegans Caenorhabditis elegans Proteins DNA-Binding Proteins Longevity Oxidative Stress Proteasome Endopeptidase Complex Pyruvaldehyde Signal Transduction Threonine Transcription Factors Impairing L-Threonine Catabolism Promotes Healthspan through Methylglyoxal-Mediated Proteohormesis. http://purl.org/coar/resource_type/c_6501