Abrami L Denhardt-Eriksson RA Hatzimanikatis V van der Goot FG 2019 Proteins can be radiolabeled either during synthesis, typically using 35S-cysteine/methionine (35S-Cys/Met), or after synthesis, by adding a radiolabeled posttranslational modification. Here we describe how protein S-palmitoylation, and its dynamics, can be monitored by 3H-palmitate labeling and how the importance of S-palmitoylation in protein biogenesis and turnover can be investigated using 35S-Cys/Met pulse-chase metabolic labeling. Proteins frequently have multiple palmitoylation sites. The importance thereof on the design and interpretation of metabolic labeling experiments is discussed. https://sonar.ch/global/documents/226055 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-9532-5_9 info:eu-repo/semantics/altIdentifier/pmid/31152399 Methods in molecular biology (Clifton, N.J.). - 2019 Data-driven mathematical modeling Metabolic labeling Protein S-acyltransferase (PAT) Pulse–chase Radiolabeling S-palmitoylation Turnover Cell Line, Transformed Humans Isotope Labeling Lipoylation Palmitic Acid Protein Processing, Post-Translational Sulfur Radioisotopes Dynamic Radiolabeling of S-Palmitoylated Proteins. http://purl.org/coar/resource_type/c_6501