<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Souverain S</dc:creator>
  <dc:creator>Rudaz S</dc:creator>
  <dc:creator>Veuthey JL</dc:creator>
  <dc:date>2004</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Three protein precipitation (PP) procedures with acetonitrile (ACN), perchloric acid (PA) and trichloroacetic acid (TCA) were investigated for the analysis of a drug cocktail from human plasma samples containing three pharmaceutical compounds and their primary metabolites. For this purpose, a capillary liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) method was developed for the simultaneous analysis of the six tested compounds in less than 6 min. Matrix effect was tested for each PP procedure by means of a post-column infusion system. The three PP techniques were found effective in removing proteins from human plasma and were fully compatible with capillary LC-ESI-MS analysis. However, with acid precipitations, low analyte recovery and a high variability, probably due to analyte coprecipitation, were obtained. Finally, ACN was found to be the most effective PP technique with a recovery higher than 80% and CV inferior to 6%.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/230495</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpba.2004.03.005</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/15193736</dc:relation>
  <dc:source>Journal of pharmaceutical and biomedical analysis. - 2004</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Blood Proteins</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Chemical Precipitation</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Chromatography, Liquid</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Spectrometry, Mass, Electrospray Ionization</dc:subject>
  <dc:title xmlns:ns6="xml" ns6:lang="en">Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>