Courvoisier S Saklayen N Huber M Chen J Diebold ED Bonacina L Wolf JP Mazur E 2015 Improving the efficiency, cell survival, and throughput of methods to modify and control the genetic expression of cells is of great benefit to biology and medicine. We investigate, both computationally and experimentally, a nanostructured substrate made of tipless pyramids for plasmonic-induced transfection. By optimizing the geometrical parameters for an excitation wavelength of 800 nm, we demonstrate a 100-fold intensity enhancement of the electric near field at the cell-substrate contact area, while the low absorption typical for gold is maintained. We demonstrate that such a substrate can induce transient poration of cells by a purely optically induced process. https://sonar.ch/global/documents/23143 eng info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.nanolett.5b01697 info:eu-repo/semantics/altIdentifier/pmid/26079771 Nano letters. - 2015 Cell transfection nanophotonics nanostructured substrate plasmonics tipless pyramid ultrafast laser Cell Survival Gold HeLa Cells Humans Lasers Nanostructures Optics and Photonics Surface Plasmon Resonance Transfection Plasmonic Tipless Pyramid Arrays for Cell Poration. http://purl.org/coar/resource_type/c_6501