Schmidt A Belaaouaj A Bissinger R Koller G Malleret L D'Orazio C Facchinelli M Schulte-Hubbert B Molinaro A Holst O Hammermann J Schniederjans M Meyer KC Damkiaer S Piacentini G Assael B Bruce K Häußler S LiPuma JJ Seelig J Worlitzsch D Döring G 2014 BACKGROUND How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. METHODS We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. RESULTS Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes. CONCLUSION Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF. application/pdf https://sonar.ch/global/documents/232267 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcf.2014.03.004 info:eu-repo/semantics/altIdentifier/pmid/24713593 info:eu-repo/semantics/openAccess Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society. - 2014 Inflammation Neutrophil elastase Pseudomonas aeruginosa Temperature Adolescent Animals Body Temperature Case-Control Studies Child Cystic Fibrosis Disease Models, Animal Female Hot Temperature Humans Leukocyte Elastase Male Mice Mice, Inbred C57BL Pseudomonas Infections Pseudomonas aeruginosa Respiratory Tract Infections Neutrophil elastase-mediated increase in airway temperature during inflammation. http://purl.org/coar/resource_type/c_6501