Liu CH Finke A Díaz M Rozhon W Poppenberger B Baubec T Pecinka A 2015 DNA damage repair is an essential cellular mechanism that maintains genome stability. Here, we show that the nonmethylable cytidine analog zebularine induces a DNA damage response in Arabidopsis thaliana, independent of changes in DNA methylation. In contrast to genotoxic agents that induce damage in a cell cycle stage-independent manner, zebularine induces damage specifically during strand synthesis in DNA replication. The signaling of this damage is mediated by additive activity of ATAXIA TELANGIECTASIA MUTATED AND RAD3-RELATED and ATAXIA TELANGIECTASIA MUTATED kinases, which cause postreplicative cell cycle arrest and increased endoreplication. The repair requires a functional STRUCTURAL MAINTENANCE OF CHROMOSOMES5 (SMC5)-SMC6 complex and is accomplished predominantly by synthesis-dependent strand-annealing homologous recombination. Here, we provide insight into the response mechanism for coping with the genotoxic effects of zebularine and identify several components of the zebularine-induced DNA damage repair pathway. application/pdf https://sonar.ch/global/documents/235777 eng info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.114.135467 info:eu-repo/semantics/altIdentifier/pmid/26023162 info:eu-repo/semantics/openAccess The Plant cell. - 2015 Arabidopsis Arabidopsis Proteins Ataxia Telangiectasia Mutated Proteins Cell Cycle Cytidine DNA Damage DNA Repair DNA Replication Repair of DNA Damage Induced by the Cytidine Analog Zebularine Requires ATR and ATM in Arabidopsis. http://purl.org/coar/resource_type/c_6501