Rohrer S Berger-Bächi B 2003 Protein-protein interactions play an important role in all cellular processes. The development of two-hybrid systems in yeast and bacteria allows for in vivo assessment of such interactions. Using a recently developed bacterial two-hybrid system, the interactions of the Staphylococcus aureus proteins FemA, FemB and FmhB, members of the FemABX protein family, which is involved in peptidoglycan biosynthesis and beta-lactam resistance of numerous Gram-positive bacteria, were analysed. While FmhB is involved in the addition of glycine 1 of the pentaglycine interpeptide of S. aureus peptidoglycan, FemA and FemB are specific for glycines 2/3 and 4/5, respectively. FemA-FemA, FemA-FemB and FemB-FemB interactions were found, while FmhB exists solely as a monomer. Interactions detected by the bacterial two-hybrid system were confirmed using the glutathione S-transferase-pulldown assay and gel filtration. application/pdf https://sonar.ch/global/documents/235965 eng info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.26315-0 info:eu-repo/semantics/altIdentifier/pmid/14523106 info:eu-repo/semantics/openAccess Microbiology (Reading, England). - 2003 Bacterial Proteins Chromatography, Gel Dimerization Molecular Weight Staphylococcus Two-Hybrid System Techniques Application of a bacterial two-hybrid system for the analysis of protein-protein interactions between FemABX family proteins. http://purl.org/coar/resource_type/c_6501