Kovtonyuk LV Manz MG Takizawa H 2016 In steady-state adult hematopoiesis, most hematopoietic stem cells (HSCs) are in the resting phase of the cell cycle. Upon enhanced hematopoietic demand, HSCs can be induced to divide and self-renew or differentiate. However, the cell-extrinsic signals inducing HSC cycling remain to be elucidated. Using in vivo high-resolution single HSC divisional tracking, we directly demonstrate that clinically applied thrombopoietin receptor but not granulocyte colony-stimulating factor (G-CSF) receptor agonists drive HSCs into self-renewing divisions leading to quantitative expansion of functional HSC as defined by their in vivo serial multilineage and long-term repopulating potential. These results suggest that thrombopoietin mimetics might be applicable to expand HSCs in vivo and to sensitize thrombopoietin receptor-expressing HSCs to cell cycle-dependent cytotoxic agents. application/pdf https://sonar.ch/global/documents/236003 eng info:eu-repo/semantics/altIdentifier/doi/10.1182/blood-2015-09-669929 info:eu-repo/semantics/altIdentifier/pmid/27146433 info:eu-repo/semantics/openAccess Blood. - 2016 Animals Cell Division Cells, Cultured Granulocyte Colony-Stimulating Factor Hematopoiesis Hematopoietic Stem Cells Heterocyclic Compounds Humans Mice Mice, Inbred C57BL Mice, Transgenic Receptors, Fc Receptors, Granulocyte Colony-Stimulating Factor Receptors, Thrombopoietin Recombinant Fusion Proteins Thrombopoietin Enhanced thrombopoietin but not G-CSF receptor stimulation induces self-renewing hematopoietic stem cell divisions in vivo. http://purl.org/coar/resource_type/c_6501