Hülsmeier AJ Welti M Hennet T 2011 Glycosylation is a complex form of protein modification occurring in the secretory pathway. The addition of N- and O-glycans affects intracellular processes like the folding and trafficking of most glycoproteins. To better understand the impact of glycosylation in protein folding and maturation, parameters like glycosylation site occupancy and oligosaccharide structure must be measured quantitatively. In this chapter, we describe current methods enabling the determination of N-glycosylation by assessment of cellular dolichol phosphate levels, dolichol-linked oligosaccharides, and the occupancy of N-glycosylation sites. We also provide detailed methods for the analysis of O-glycosylation, whose role in intracellular protein maturation is often overlooked. application/pdf https://sonar.ch/global/documents/236036 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-385928-0.00010-9 info:eu-repo/semantics/altIdentifier/pmid/21329800 info:eu-repo/semantics/openAccess Methods in enzymology. - 2011 Animals Chromatography, High Pressure Liquid Dolichol Phosphates Dolichols Glycoproteins Glycosylation Humans Oligosaccharides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Unfolded Protein Response Glycoprotein maturation and the UPR. http://purl.org/coar/resource_type/c_6501