<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Lescat M</dc:creator>
  <dc:creator>Poirel L</dc:creator>
  <dc:creator>Nordmann P</dc:creator>
  <dc:date>2018</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">A rapid (total time &lt;2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr-1 and mcr-5 genes.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/2427</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2018.04.010</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/30220493</dc:relation>
  <dc:source>Diagnostic microbiology and infectious disease. - 2018</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Multiplex PCR</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Polymyxin resistance6</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">mcr genes</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Anti-Bacterial Agents</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Bacteria</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Drug Resistance, Bacterial</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Escherichia coli Proteins</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Genes, Bacterial</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Microbial Sensitivity Tests</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Multiplex Polymerase Chain Reaction</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Sensitivity and Specificity</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Transferases (Other Substituted Phosphate Groups)</dc:subject>
  <dc:title xmlns:ns14="xml" ns14:lang="en">Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>