Valkov E Jonas S Weichenrieder O 2017 Cellular mRNA levels are regulated via rates of transcription and decay. Since the removal of the mRNA 5'-cap by the decapping enzyme DCP2 is generally an irreversible step towards decay, it requires regulation. Control of DCP2 activity is likely effected by two interdependent means: by conformational control of the DCP2-DCP1 complex, and by assembly control of the decapping network, an array of mutually interacting effector proteins. Here, we compare three recent and conformationally distinct crystal structures of the DCP2-DCP1 decapping complex in the presence of substrate analogs and decapping enhancers and we discuss alternative substrate recognition modes for the catalytic domain of DCP2. Together with structure-based insight into decapping network assembly, we propose that DCP2-mediated decapping follows more than one path. https://sonar.ch/global/documents/253694 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2017.05.009 info:eu-repo/semantics/altIdentifier/pmid/28591671 Current opinion in structural biology. - 2017 Catalysis Endoribonucleases Eukaryota Hydrolysis Models, Molecular Molecular Conformation RNA Caps RNA Stability RNA, Messenger Structure-Activity Relationship Mille viae in eukaryotic mRNA decapping. http://purl.org/coar/resource_type/c_6501